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KMID : 0374019940170010003
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Ewha Medical Journal
1994 Volume.17 No. 1 p.3 ~ p.12
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Comparative Study of Flow Cytometry and Image Cytometry of Hepatocellular Carcinomas in Human and Experimental Aminal Tissue
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Abstract
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The rapidly expanding field of flow cytometry consists of the simultaneous measurement of several parameters while a suspension of cells flows through a beam of light past stationary detectors.
Flow cytometric DNA analysis can even be performed on nuclear suspensions recovered from thick sections of routine formalin-fixed, paraffin-embedded tissue blocks with results remarkably similar to those obtained using fresh tissues.
Because the main limitation of flow cytometry is that cells need to be in a single-cell suspension in order to be analysed, image cytometric device was recentry developed.
At present study, we did comparison between flow cytometric and image cytometric methods in measurement of DNA ploidy pattern of same specimen from human and experimental animal tissue and obtained a good correlation between 2 methods.
It is concluded that DNA image cytometry appears to be supplementary to FCM for the study of DNA ploidy abnormalities and that the combined results of these methods have a major influence on the clinical outcome.
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KEYWORD
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